Effect of Hepatic Microsomal Enzyme Inducers on the Phenobarbitone Sleeping Time in Mice

Aim: To study of effect of hepatic microsomal enzyme inducers on the phenobarbitone sleeping time in mice.

Principle

Hypnosis is a natural sleep process, and substances or medications that induce it are referred to as hypnotic agents. While this concept primarily applies to human patients, in animal experiments, the term "hypnotic" is used to describe a deeper stage of central depression that leads to unconsciousness, accompanied by the loss of righting reflexes and muscle tone. 

The term "loss of righting reflex" is commonly used to describe the animal's state of "sleep," characterized by the inability to correct its posture when placed on its back. Normally, animals instinctively turn their bodies so that their paws or feet are positioned towards the ground—an action known as the righting reflex. The righting reflex relies on the proper functioning of the vestibular, visual, and proprioceptive systems. 

Materials and Methods
Materials
Animal/species :     Mouse/Swiss albino
Sex/Body weight :    Either sex/ 20-30 g
Syringe/needle : 1ml/ preferably 24G onwards
Drug : Phenobarbitone (80 mg/kg, i.p)

Methods

Step 1
• Weigh the animals and mark them properly to distinguish from one another

• Divide animals into two groups (n = 6 in each group)

Step 2
• Group 1: Control group (n = 6); mice are given saline at the equivalent dose of drug
• Group 2: Treatment group (n = 6); mice are given Phenobarbitone (80 mg/kg, i.p).

Step 3
• Observe the animals for 45 min to 1 hour
• Observe for the onset of loss of righting reflex and duration of loss of righting reflex

Observations

GroupDayOnset of Sleep (minutes)Duration of Sleep (minutes)
Control17, 8, 9, 11, 12, 1335, 31, 29, 40, 27, 38
Control28, 9, 10, 12, 14, 1640, 38, 36, 39, 37, 41
Control39, 9, 10, 11, 12, 1441, 39, 38, 40, 37, 36
Control410, 10, 11, 12, 13, 1541, 30, 29, 32, 27, 35
Control510, 11, 12, 14, 15, 1841, 36, 35, 38, 33, 39
Treatment13, 4, 5, 5, 5, 545, 48, 49, 51, 53, 54
Treatment24, 4, 5, 6, 6, 645, 48, 49, 51, 53, 55
Treatment35, 5, 6, 7, 7, 745, 47, 48, 49, 51, 53
Treatment46, 7, 7, 8, 8, 845, 46, 47, 49, 51, 53
Treatment57, 8, 8, 9, 9, 943, 45, 46, 48, 49, 52

 

Please note that the data provided is fictional and serves as an example. The actual values may vary based on the specific experimental conditions, the enzyme inducer used, and other factors. Additionally, statistical analysis should be performed to assess the significance of any observed differences between the control and treatment groups.

Step 4

  • Data Analysis: Analyze the data by comparing the sleeping time of the control group with the treatment groups. Statistical tests such as t-tests or analysis of variance (ANOVA) can be used to determine significant differences between groups.
  • Interpretation: Based on the results, draw conclusions regarding the effect of hepatic microsomal enzyme inducers on the phenobarbitone sleeping time in mice. Discuss any potential implications and mechanisms underlying the observed effects.

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