Study of Effect of Drugs on Ciliary Motility of Frog Esophagus

Introduction

The study aims to investigate the effect of drugs on the ciliary motility of the frog esophagus. Ciliary motility refers to the rhythmic and coordinated movement of cilia, which are hair-like structures present on the surface of cells in the esophagus. The ciliary movement plays a crucial role in transporting mucus and other substances along the esophageal lining.

Requirements

Animal: Frog

Drugs: Acetylcholine, Physostigmine, Atropine

Physiological Salt Solution: Frog's Ringer Solution

Equipment: Frog board, and Poppy seeds

Procedure

  1. Decapitate the frog and pin the frog to the frog board on its back.
  2. Pin the lower jaw to the abdomen cutting sufficiently the buccal cavity and exposing the esophagus. Keep the buccal cavity and the opening of the esophagus wet by irrigating it with Ringer solution.
  3. Fix two parts i.e., first the beginning point at the beginning of the esophagus and the endpoint. Keep this distance constant to measure the time taken by the poppy seed to move from the beginning point to the endpoint.
  4. Place a poppy seed at the beginning point. Turn on the stopwatch and note the time taken by the seed to reach the endpoint. Repeat this several times.
  5. Put a few drops of acetylcholine on the esophagus and repeat step 4. Note the time.
  6. Wash the buccal cavity with Ringer solution. Put a few drops of physostigmine on the esophagus and repeat step 4. Note the time.
  7. Wash the esophagus with Ringer solution. Put a few drops of atropine on the esophagus and repeat step 4. Note the time.

Video of Experiment

Observation

Treatment

Approximate Distance

Observations (sec)

Mean (sec)

1

2

3

4

Ringer solution

4 cm27283134

Acetylcholine

4 cm20222526

Physostigmine

4 cm18202324

Atropine

4 cm35    363840

Inference

Acetylcholine, Physostigmine reduce and atropine enhances the time taken by the poppy seeds to move from the beginning point to the endpoint. 

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